| Round 1 |
| Saturday, 12 October 2024 |
| 1 | Aspull Development XV | v | Bye |
| 2 | Liverpool St Helens 2nd XV | v | Bye |
| 3 | Widnes 2nd XV | v | Bye |
| 4 | Preston Grasshoppers 4th XV | v | Bye |
| 5 | Broughton Park 2nd XV | v | Congleton |
| 6 | Northwich 2nd XV | v | Burnage 2nd XV |
| 7 | Chester 3rd XV | v | Bye |
| 8 | Holmes Chapel | v | Prenton |
| 9 | West Park (St Helens) 2nd XV | v | Littleborough 2nd XV |
| 10 | Crewe & Nantwich 2nd XV | v | St Edward's O.B. |
| 11 | Burnley 2nd XV | v | Blackpool |
| 12 | Tarleton 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| 13 | Southport 2nd XV | v | Bye |
| 14 | Sandbach 3rd XV | v | Bye |
| 15 | Sedgley Park 3rd XV | v | Bye |
| 16 | Bowdon 2nd XV | v | Didsbury Toc H 2nd XV |
| Round 2 |
| Saturday, 2 November 2024 |
| Aspull Development XV | v | Liverpool St Helens 2nd XV |
| Widnes 2nd XV | v | Preston Grasshoppers 4th XV |
| Winner of Match 5 | v | Winner of Match 6 |
| Chester 3rd XV | v | Winner of Match 8 |
| Winner of Match 9 | v | Winner of Match 10 |
| Winner of Match 11 | v | Winner of Match 12 |
| Southport 2nd XV | v | Sandbach 3rd XV |
| Sedgley Park 3rd XV | v | Winner of Match 16 |
| Round 3 |
| Saturday, 25 January 2025 |
| TBC | v | TBC |
| TBC | v | TBC |
| TBC | v | TBC |
| TBC | v | TBC |
| Round 4 |
| Saturday, 29 March 2025 |
| TBC | v | TBC |
| TBC | v | TBC |
| Round 5 |
| Saturday, 3 May 2025 |
| TBC | v | TBC |