| Saturday, 12 September 2026 |
| Aspull Development XV | v | Preston Grasshoppers 3rd XV |
| Eccles 2nd XV | v | Littleborough 2nd XV |
| Firwood Waterloo 2nd XV | v | Southport 2nd XV |
| Garstang Blues (2nd XV) | v | Tarleton 2nd XV |
| Wigan 2nd XV | v | Aldwinians 2nd XV |
| Saturday, 19 September 2026 |
| Aldwinians 2nd XV | v | Littleborough 2nd XV |
| Preston Grasshoppers 3rd XV | v | Garstang Blues (2nd XV) |
| Southport 2nd XV | v | Liverpool St Helens 2nd XV |
| Tarleton 2nd XV | v | Firwood Waterloo 2nd XV |
| Wigan 2nd XV | v | Aspull Development XV |
| Saturday, 26 September 2026 |
| Aspull Development XV | v | Aldwinians 2nd XV |
| Eccles 2nd XV | v | Southport 2nd XV |
| Firwood Waterloo 2nd XV | v | Preston Grasshoppers 3rd XV |
| Garstang Blues (2nd XV) | v | Wigan 2nd XV |
| Liverpool St Helens 2nd XV | v | Tarleton 2nd XV |
| Saturday, 3 October 2026 |
| Aspull Development XV | v | Garstang Blues (2nd XV) |
| Preston Grasshoppers 3rd XV | v | Liverpool St Helens 2nd XV |
| Southport 2nd XV | v | Littleborough 2nd XV |
| Tarleton 2nd XV | v | Eccles 2nd XV |
| Wigan 2nd XV | v | Firwood Waterloo 2nd XV |
| Saturday, 17 October 2026 |
| Eccles 2nd XV | v | Preston Grasshoppers 3rd XV |
| Firwood Waterloo 2nd XV | v | Aspull Development XV |
| Garstang Blues (2nd XV) | v | Aldwinians 2nd XV |
| Littleborough 2nd XV | v | Tarleton 2nd XV |
| Liverpool St Helens 2nd XV | v | Wigan 2nd XV |
| Saturday, 24 October 2026 |
| Aldwinians 2nd XV | v | Southport 2nd XV |
| Aspull Development XV | v | Liverpool St Helens 2nd XV |
| Garstang Blues (2nd XV) | v | Firwood Waterloo 2nd XV |
| Preston Grasshoppers 3rd XV | v | Littleborough 2nd XV |
| Wigan 2nd XV | v | Eccles 2nd XV |
| Saturday, 31 October 2026 |
| Eccles 2nd XV | v | Aspull Development XV |
| Firwood Waterloo 2nd XV | v | Aldwinians 2nd XV |
| Littleborough 2nd XV | v | Wigan 2nd XV |
| Liverpool St Helens 2nd XV | v | Garstang Blues (2nd XV) |
| Southport 2nd XV | v | Tarleton 2nd XV |
| Saturday, 7 November 2026 |
| Aldwinians 2nd XV | v | Tarleton 2nd XV |
| Aspull Development XV | v | Littleborough 2nd XV |
| Firwood Waterloo 2nd XV | v | Liverpool St Helens 2nd XV |
| Garstang Blues (2nd XV) | v | Eccles 2nd XV |
| Preston Grasshoppers 3rd XV | v | Southport 2nd XV |
| Saturday, 28 November 2026 |
| Aldwinians 2nd XV | v | Preston Grasshoppers 3rd XV |
| Aspull Development XV | v | Southport 2nd XV |
| Firwood Waterloo 2nd XV | v | Littleborough 2nd XV |
| Liverpool St Helens 2nd XV | v | Eccles 2nd XV |
| Wigan 2nd XV | v | Tarleton 2nd XV |
| Saturday, 5 December 2026 |
| Eccles 2nd XV | v | Aldwinians 2nd XV |
| Littleborough 2nd XV | v | Liverpool St Helens 2nd XV |
| Preston Grasshoppers 3rd XV | v | Wigan 2nd XV |
| Southport 2nd XV | v | Garstang Blues (2nd XV) |
| Tarleton 2nd XV | v | Aspull Development XV |
| Saturday, 12 December 2026 |
| Aspull Development XV | v | Wigan 2nd XV |
| Firwood Waterloo 2nd XV | v | Tarleton 2nd XV |
| Garstang Blues (2nd XV) | v | Preston Grasshoppers 3rd XV |
| Littleborough 2nd XV | v | Aldwinians 2nd XV |
| Liverpool St Helens 2nd XV | v | Southport 2nd XV |
| Saturday, 16 January 2027 |
| Eccles 2nd XV | v | Tarleton 2nd XV |
| Firwood Waterloo 2nd XV | v | Wigan 2nd XV |
| Garstang Blues (2nd XV) | v | Aspull Development XV |
| Littleborough 2nd XV | v | Southport 2nd XV |
| Liverpool St Helens 2nd XV | v | Preston Grasshoppers 3rd XV |
| Saturday, 23 January 2027 |
| Aldwinians 2nd XV | v | Garstang Blues (2nd XV) |
| Aspull Development XV | v | Firwood Waterloo 2nd XV |
| Preston Grasshoppers 3rd XV | v | Eccles 2nd XV |
| Tarleton 2nd XV | v | Littleborough 2nd XV |
| Wigan 2nd XV | v | Liverpool St Helens 2nd XV |
| Saturday, 6 February 2027 |
| Eccles 2nd XV | v | Wigan 2nd XV |
| Firwood Waterloo 2nd XV | v | Garstang Blues (2nd XV) |
| Littleborough 2nd XV | v | Preston Grasshoppers 3rd XV |
| Liverpool St Helens 2nd XV | v | Aspull Development XV |
| Southport 2nd XV | v | Aldwinians 2nd XV |
| Saturday, 13 February 2027 |
| Aldwinians 2nd XV | v | Firwood Waterloo 2nd XV |
| Aspull Development XV | v | Eccles 2nd XV |
| Garstang Blues (2nd XV) | v | Liverpool St Helens 2nd XV |
| Tarleton 2nd XV | v | Southport 2nd XV |
| Wigan 2nd XV | v | Littleborough 2nd XV |
| Saturday, 20 February 2027 |
| Eccles 2nd XV | v | Garstang Blues (2nd XV) |
| Littleborough 2nd XV | v | Aspull Development XV |
| Liverpool St Helens 2nd XV | v | Firwood Waterloo 2nd XV |
| Southport 2nd XV | v | Preston Grasshoppers 3rd XV |
| Tarleton 2nd XV | v | Aldwinians 2nd XV |
| Saturday, 27 February 2027 |
| Aldwinians 2nd XV | v | Aspull Development XV |
| Preston Grasshoppers 3rd XV | v | Firwood Waterloo 2nd XV |
| Southport 2nd XV | v | Eccles 2nd XV |
| Tarleton 2nd XV | v | Liverpool St Helens 2nd XV |
| Wigan 2nd XV | v | Garstang Blues (2nd XV) |
| Saturday, 6 March 2027 |
| Aldwinians 2nd XV | v | Liverpool St Helens 2nd XV |
| Firwood Waterloo 2nd XV | v | Eccles 2nd XV |
| Garstang Blues (2nd XV) | v | Littleborough 2nd XV |
| Preston Grasshoppers 3rd XV | v | Tarleton 2nd XV |
| Wigan 2nd XV | v | Southport 2nd XV |
| Saturday, 13 March 2027 |
| Eccles 2nd XV | v | Liverpool St Helens 2nd XV |
| Littleborough 2nd XV | v | Firwood Waterloo 2nd XV |
| Preston Grasshoppers 3rd XV | v | Aldwinians 2nd XV |
| Southport 2nd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Wigan 2nd XV |
| Saturday, 3 April 2027 |
| Aldwinians 2nd XV | v | Eccles 2nd XV |
| Aspull Development XV | v | Tarleton 2nd XV |
| Garstang Blues (2nd XV) | v | Southport 2nd XV |
| Liverpool St Helens 2nd XV | v | Littleborough 2nd XV |
| Wigan 2nd XV | v | Preston Grasshoppers 3rd XV |
| Saturday, 10 April 2027 |
| Aldwinians 2nd XV | v | Wigan 2nd XV |
| Littleborough 2nd XV | v | Eccles 2nd XV |
| Preston Grasshoppers 3rd XV | v | Aspull Development XV |
| Southport 2nd XV | v | Firwood Waterloo 2nd XV |
| Tarleton 2nd XV | v | Garstang Blues (2nd XV) |
| Saturday, 17 April 2027 |
| Eccles 2nd XV | v | Firwood Waterloo 2nd XV |
| Littleborough 2nd XV | v | Garstang Blues (2nd XV) |
| Liverpool St Helens 2nd XV | v | Aldwinians 2nd XV |
| Southport 2nd XV | v | Wigan 2nd XV |
| Tarleton 2nd XV | v | Preston Grasshoppers 3rd XV |