| Saturday, 12 September 2026 |
| Birkenhead Park Wanderers (2nd XV) | v | Anselmians 2nd XV |
| Blackburn 2nd XV | v | West Park (St Helens) 2nd XV |
| Ellesmere Port | v | Widnes 2nd XV |
| Kendal 2nd XV | v | Fylde Vandals |
| Kirkby Lonsdale RUFC Development XV | v | Rochdale 2nd XV |
| Manchester 2nd XV | v | Vale of Lune 2nd XV |
| Saturday, 19 September 2026 |
| Anselmians 2nd XV | v | Vale of Lune 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Blackburn 2nd XV |
| Fylde Vandals | v | Kirkby Lonsdale RUFC Development XV |
| Rochdale 2nd XV | v | Manchester 2nd XV |
| West Park (St Helens) 2nd XV | v | Ellesmere Port |
| Widnes 2nd XV | v | Kendal 2nd XV |
| Saturday, 26 September 2026 |
| Blackburn 2nd XV | v | Anselmians 2nd XV |
| Ellesmere Port | v | Birkenhead Park Wanderers (2nd XV) |
| Kendal 2nd XV | v | West Park (St Helens) 2nd XV |
| Manchester 2nd XV | v | Fylde Vandals |
| Vale of Lune 2nd XV | v | Rochdale 2nd XV |
| Widnes 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 3 October 2026 |
| Birkenhead Park Wanderers (2nd XV) | v | Kendal 2nd XV |
| Blackburn 2nd XV | v | Ellesmere Port |
| Fylde Vandals | v | Vale of Lune 2nd XV |
| Manchester 2nd XV | v | Widnes 2nd XV |
| Rochdale 2nd XV | v | Anselmians 2nd XV |
| West Park (St Helens) 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 17 October 2026 |
| Birkenhead Park Wanderers (2nd XV) | v | Kirkby Lonsdale RUFC Development XV |
| Ellesmere Port | v | Anselmians 2nd XV |
| Kendal 2nd XV | v | Blackburn 2nd XV |
| Manchester 2nd XV | v | West Park (St Helens) 2nd XV |
| Rochdale 2nd XV | v | Fylde Vandals |
| Vale of Lune 2nd XV | v | Widnes 2nd XV |
| Saturday, 24 October 2026 |
| Anselmians 2nd XV | v | Fylde Vandals |
| Birkenhead Park Wanderers (2nd XV) | v | Manchester 2nd XV |
| Blackburn 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Ellesmere Port | v | Kendal 2nd XV |
| West Park (St Helens) 2nd XV | v | Vale of Lune 2nd XV |
| Widnes 2nd XV | v | Rochdale 2nd XV |
| Saturday, 31 October 2026 |
| Fylde Vandals | v | Widnes 2nd XV |
| Kendal 2nd XV | v | Anselmians 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Ellesmere Port |
| Manchester 2nd XV | v | Blackburn 2nd XV |
| Rochdale 2nd XV | v | West Park (St Helens) 2nd XV |
| Vale of Lune 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Saturday, 7 November 2026 |
| Anselmians 2nd XV | v | Widnes 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Rochdale 2nd XV |
| Blackburn 2nd XV | v | Vale of Lune 2nd XV |
| Ellesmere Port | v | Manchester 2nd XV |
| Kendal 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| West Park (St Helens) 2nd XV | v | Fylde Vandals |
| Saturday, 28 November 2026 |
| Anselmians 2nd XV | v | West Park (St Helens) 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Widnes 2nd XV |
| Blackburn 2nd XV | v | Fylde Vandals |
| Ellesmere Port | v | Rochdale 2nd XV |
| Kendal 2nd XV | v | Vale of Lune 2nd XV |
| Manchester 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 5 December 2026 |
| Fylde Vandals | v | Ellesmere Port |
| Manchester 2nd XV | v | Anselmians 2nd XV |
| Rochdale 2nd XV | v | Kendal 2nd XV |
| Vale of Lune 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| West Park (St Helens) 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Widnes 2nd XV | v | Blackburn 2nd XV |
| Saturday, 12 December 2026 |
| Blackburn 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Ellesmere Port | v | West Park (St Helens) 2nd XV |
| Kendal 2nd XV | v | Widnes 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Fylde Vandals |
| Manchester 2nd XV | v | Rochdale 2nd XV |
| Vale of Lune 2nd XV | v | Anselmians 2nd XV |
| Saturday, 9 January 2027 |
| Kirkby Lonsdale RUFC Development XV | v | Widnes 2nd XV |
| Saturday, 16 January 2027 |
| Anselmians 2nd XV | v | Rochdale 2nd XV |
| Ellesmere Port | v | Blackburn 2nd XV |
| Kendal 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Kirkby Lonsdale RUFC Development XV | v | West Park (St Helens) 2nd XV |
| Vale of Lune 2nd XV | v | Fylde Vandals |
| Widnes 2nd XV | v | Manchester 2nd XV |
| Saturday, 23 January 2027 |
| Anselmians 2nd XV | v | Ellesmere Port |
| Blackburn 2nd XV | v | Kendal 2nd XV |
| Fylde Vandals | v | Rochdale 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Birkenhead Park Wanderers (2nd XV) |
| West Park (St Helens) 2nd XV | v | Manchester 2nd XV |
| Widnes 2nd XV | v | Vale of Lune 2nd XV |
| Saturday, 6 February 2027 |
| Fylde Vandals | v | Anselmians 2nd XV |
| Kendal 2nd XV | v | Ellesmere Port |
| Kirkby Lonsdale RUFC Development XV | v | Blackburn 2nd XV |
| Manchester 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Rochdale 2nd XV | v | Widnes 2nd XV |
| Vale of Lune 2nd XV | v | West Park (St Helens) 2nd XV |
| Saturday, 13 February 2027 |
| Anselmians 2nd XV | v | Kendal 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Vale of Lune 2nd XV |
| Blackburn 2nd XV | v | Manchester 2nd XV |
| Ellesmere Port | v | Kirkby Lonsdale RUFC Development XV |
| West Park (St Helens) 2nd XV | v | Rochdale 2nd XV |
| Widnes 2nd XV | v | Fylde Vandals |
| Saturday, 20 February 2027 |
| Fylde Vandals | v | West Park (St Helens) 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Kendal 2nd XV |
| Manchester 2nd XV | v | Ellesmere Port |
| Rochdale 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Vale of Lune 2nd XV | v | Blackburn 2nd XV |
| Widnes 2nd XV | v | Anselmians 2nd XV |
| Saturday, 27 February 2027 |
| Anselmians 2nd XV | v | Blackburn 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Ellesmere Port |
| Fylde Vandals | v | Manchester 2nd XV |
| Rochdale 2nd XV | v | Vale of Lune 2nd XV |
| West Park (St Helens) 2nd XV | v | Kendal 2nd XV |
| Saturday, 6 March 2027 |
| Anselmians 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Birkenhead Park Wanderers (2nd XV) | v | Fylde Vandals |
| Blackburn 2nd XV | v | Rochdale 2nd XV |
| Ellesmere Port | v | Vale of Lune 2nd XV |
| Kendal 2nd XV | v | Manchester 2nd XV |
| West Park (St Helens) 2nd XV | v | Widnes 2nd XV |
| Saturday, 13 March 2027 |
| Fylde Vandals | v | Blackburn 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Manchester 2nd XV |
| Rochdale 2nd XV | v | Ellesmere Port |
| Vale of Lune 2nd XV | v | Kendal 2nd XV |
| West Park (St Helens) 2nd XV | v | Anselmians 2nd XV |
| Widnes 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Saturday, 3 April 2027 |
| Anselmians 2nd XV | v | Manchester 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | West Park (St Helens) 2nd XV |
| Blackburn 2nd XV | v | Widnes 2nd XV |
| Ellesmere Port | v | Fylde Vandals |
| Kendal 2nd XV | v | Rochdale 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Vale of Lune 2nd XV |
| Saturday, 10 April 2027 |
| Anselmians 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Fylde Vandals | v | Kendal 2nd XV |
| Rochdale 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Vale of Lune 2nd XV | v | Manchester 2nd XV |
| West Park (St Helens) 2nd XV | v | Blackburn 2nd XV |
| Widnes 2nd XV | v | Ellesmere Port |
| Saturday, 17 April 2027 |
| Fylde Vandals | v | Birkenhead Park Wanderers (2nd XV) |
| Kirkby Lonsdale RUFC Development XV | v | Anselmians 2nd XV |
| Manchester 2nd XV | v | Kendal 2nd XV |
| Rochdale 2nd XV | v | Blackburn 2nd XV |
| Vale of Lune 2nd XV | v | Ellesmere Port |
| Widnes 2nd XV | v | West Park (St Helens) 2nd XV |