| Saturday, 7 September 2024 |
| Liverpool St Helens 2nd XV | Postponed | Kirkby Lonsdale RUFC Development XV |
| Preston Grasshoppers 4th XV | 36 | 21 | Aspull Development XV |
| Southport 2nd XV | 31 | 35 | Blackpool |
| Tarleton 2nd XV | 19 | 33 | Widnes 2nd XV |
| West Park (St Helens) 2nd XV | 64 | 7 | Burnley 2nd XV |
| Saturday, 14 September 2024 |
| Blackpool | v | Liverpool St Helens 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Preston Grasshoppers 4th XV |
| Littleborough 2nd XV | v | Burnley 2nd XV |
| Sedgley Park 3rd XV | v | Southport 2nd XV |
| West Park (St Helens) 2nd XV | v | Tarleton 2nd XV |
| Saturday, 21 September 2024 |
| Burnley 2nd XV | v | Aspull Development XV |
| Liverpool St Helens 2nd XV | v | Sedgley Park 3rd XV |
| Preston Grasshoppers 4th XV | v | Blackpool |
| Tarleton 2nd XV | v | Littleborough 2nd XV |
| Widnes 2nd XV | v | West Park (St Helens) 2nd XV |
| Saturday, 28 September 2024 |
| Aspull Development XV | v | Tarleton 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Burnley 2nd XV |
| Littleborough 2nd XV | v | Widnes 2nd XV |
| Liverpool St Helens 2nd XV | v | Southport 2nd XV |
| Sedgley Park 3rd XV | v | Preston Grasshoppers 4th XV |
| Saturday, 5 October 2024 |
| Aspull Development XV | v | Sedgley Park 3rd XV |
| Blackpool | v | Kirkby Lonsdale RUFC Development XV |
| Littleborough 2nd XV | v | Southport 2nd XV |
| Preston Grasshoppers 4th XV | v | Widnes 2nd XV |
| West Park (St Helens) 2nd XV | v | Liverpool St Helens 2nd XV |
| Saturday, 12 October 2024 |
| Burnley 2nd XV | v | Blackpool |
| Preston Grasshoppers 4th XV | v | Southport 2nd XV |
| Tarleton 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| West Park (St Helens) 2nd XV | v | Littleborough 2nd XV |
| Widnes 2nd XV | v | Aspull Development XV |
| Saturday, 19 October 2024 |
| Aspull Development XV | v | West Park (St Helens) 2nd XV |
| Blackpool | v | Tarleton 2nd XV |
| Burnley 2nd XV | v | Sedgley Park 3rd XV |
| Kirkby Lonsdale RUFC Development XV | v | Widnes 2nd XV |
| Liverpool St Helens 2nd XV | v | Preston Grasshoppers 4th XV |
| Saturday, 26 October 2024 |
| Burnley 2nd XV | v | Southport 2nd XV |
| Littleborough 2nd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Sedgley Park 3rd XV |
| West Park (St Helens) 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Widnes 2nd XV | v | Blackpool |
| Saturday, 9 November 2024 |
| Blackpool | v | West Park (St Helens) 2nd XV |
| Littleborough 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Liverpool St Helens 2nd XV | v | Burnley 2nd XV |
| Sedgley Park 3rd XV | v | Widnes 2nd XV |
| Tarleton 2nd XV | v | Southport 2nd XV |
| Saturday, 16 November 2024 |
| Aspull Development XV | v | Kirkby Lonsdale RUFC Development XV |
| Burnley 2nd XV | v | Preston Grasshoppers 4th XV |
| Littleborough 2nd XV | v | Blackpool |
| Sedgley Park 3rd XV | v | West Park (St Helens) 2nd XV |
| Tarleton 2nd XV | v | Liverpool St Helens 2nd XV |
| Widnes 2nd XV | v | Southport 2nd XV |
| Saturday, 23 November 2024 |
| Blackpool | v | Aspull Development XV |
| Liverpool St Helens 2nd XV | v | Widnes 2nd XV |
| Preston Grasshoppers 4th XV | v | Tarleton 2nd XV |
| Sedgley Park 3rd XV | v | Littleborough 2nd XV |
| Southport 2nd XV | v | West Park (St Helens) 2nd XV |
| Saturday, 30 November 2024 |
| Kirkby Lonsdale RUFC Development XV | v | Sedgley Park 3rd XV |
| Liverpool St Helens 2nd XV | v | Littleborough 2nd XV |
| Southport 2nd XV | v | Aspull Development XV |
| Tarleton 2nd XV | v | Burnley 2nd XV |
| West Park (St Helens) 2nd XV | v | Preston Grasshoppers 4th XV |
| Saturday, 7 December 2024 |
| Kirkby Lonsdale RUFC Development XV | v | Blackpool |
| Liverpool St Helens 2nd XV | v | West Park (St Helens) 2nd XV |
| Sedgley Park 3rd XV | v | Aspull Development XV |
| Southport 2nd XV | v | Littleborough 2nd XV |
| Widnes 2nd XV | v | Preston Grasshoppers 4th XV |
| Saturday, 14 December 2024 |
| Aspull Development XV | v | Southport 2nd XV |
| Burnley 2nd XV | v | Tarleton 2nd XV |
| Littleborough 2nd XV | v | Liverpool St Helens 2nd XV |
| Preston Grasshoppers 4th XV | v | West Park (St Helens) 2nd XV |
| Sedgley Park 3rd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 21 December 2024 |
| Aspull Development XV | v | Liverpool St Helens 2nd XV |
| Blackpool | v | Sedgley Park 3rd XV |
| Kirkby Lonsdale RUFC Development XV | v | Southport 2nd XV |
| Littleborough 2nd XV | v | Preston Grasshoppers 4th XV |
| Widnes 2nd XV | v | Burnley 2nd XV |
| Saturday, 11 January 2025 |
| Aspull Development XV | v | Burnley 2nd XV |
| Blackpool | v | Preston Grasshoppers 4th XV |
| Littleborough 2nd XV | v | Tarleton 2nd XV |
| Sedgley Park 3rd XV | v | Liverpool St Helens 2nd XV |
| West Park (St Helens) 2nd XV | v | Widnes 2nd XV |
| Saturday, 18 January 2025 |
| Burnley 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Preston Grasshoppers 4th XV | v | Sedgley Park 3rd XV |
| Southport 2nd XV | v | Liverpool St Helens 2nd XV |
| Tarleton 2nd XV | v | Aspull Development XV |
| Widnes 2nd XV | v | Littleborough 2nd XV |
| Saturday, 1 February 2025 |
| Aspull Development XV | v | Widnes 2nd XV |
| Blackpool | v | Burnley 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Tarleton 2nd XV |
| Littleborough 2nd XV | v | West Park (St Helens) 2nd XV |
| Southport 2nd XV | v | Preston Grasshoppers 4th XV |
| Saturday, 8 February 2025 |
| Burnley 2nd XV | v | Widnes 2nd XV |
| Liverpool St Helens 2nd XV | v | Aspull Development XV |
| Preston Grasshoppers 4th XV | v | Littleborough 2nd XV |
| Sedgley Park 3rd XV | v | Blackpool |
| Southport 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 15 February 2025 |
| Preston Grasshoppers 4th XV | v | Liverpool St Helens 2nd XV |
| Sedgley Park 3rd XV | v | Burnley 2nd XV |
| Tarleton 2nd XV | v | Blackpool |
| West Park (St Helens) 2nd XV | v | Aspull Development XV |
| Widnes 2nd XV | v | Kirkby Lonsdale RUFC Development XV |
| Saturday, 22 February 2025 |
| Aspull Development XV | v | Preston Grasshoppers 4th XV |
| Blackpool | v | Southport 2nd XV |
| Burnley 2nd XV | v | West Park (St Helens) 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Liverpool St Helens 2nd XV |
| Widnes 2nd XV | v | Tarleton 2nd XV |
| Saturday, 1 March 2025 |
| Aspull Development XV | v | Littleborough 2nd XV |
| Blackpool | v | Widnes 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | West Park (St Helens) 2nd XV |
| Sedgley Park 3rd XV | v | Tarleton 2nd XV |
| Southport 2nd XV | v | Burnley 2nd XV |
| Saturday, 8 March 2025 |
| Burnley 2nd XV | v | Liverpool St Helens 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Littleborough 2nd XV |
| Southport 2nd XV | v | Tarleton 2nd XV |
| West Park (St Helens) 2nd XV | v | Blackpool |
| Widnes 2nd XV | v | Sedgley Park 3rd XV |
| Saturday, 22 March 2025 |
| Blackpool | v | Littleborough 2nd XV |
| Kirkby Lonsdale RUFC Development XV | v | Aspull Development XV |
| Liverpool St Helens 2nd XV | v | Tarleton 2nd XV |
| Preston Grasshoppers 4th XV | v | Burnley 2nd XV |
| Southport 2nd XV | v | Widnes 2nd XV |
| West Park (St Helens) 2nd XV | v | Sedgley Park 3rd XV |
| Saturday, 5 April 2025 |
| Aspull Development XV | v | Blackpool |
| Littleborough 2nd XV | v | Sedgley Park 3rd XV |
| Tarleton 2nd XV | v | Preston Grasshoppers 4th XV |
| West Park (St Helens) 2nd XV | v | Southport 2nd XV |
| Widnes 2nd XV | v | Liverpool St Helens 2nd XV |
| Saturday, 26 April 2025 |
| Burnley 2nd XV | v | Littleborough 2nd XV |
| Liverpool St Helens 2nd XV | v | Blackpool |
| Preston Grasshoppers 4th XV | v | Kirkby Lonsdale RUFC Development XV |
| Southport 2nd XV | v | Sedgley Park 3rd XV |
| Tarleton 2nd XV | v | West Park (St Helens) 2nd XV |