| Round 1 |
| Saturday, 30 September 2023 |
| 1 | Aldwinians 2nd XV | 17 | 14 | Widnes 2nd XV |
| 2 | Sandbach 3rd XV | 8 | 36 | Eagle |
| 3 | Southport 2nd XV | 7 | 15 | Eccles 2nd XV |
| 4 | Garstang Blues (2nd XV) | v | Bye |
| 5 | Northwich 2nd XV | 17 | 10 | Holmes Chapel |
| 6 | Didsbury Toc H 2nd XV | v | Bye |
| 7 | Liverpool St Helens 2nd XV | v | Bye |
| 8 | Crewe & Nantwich 2nd XV | v | Bye |
| 9 | Warrington 2nd XV | v | Bye |
| 10 | Congleton | v | Bye |
| 11 | Burnage 2nd XV | v | Bye |
| 12 | Sedgley Park 3rd XV | v | Bye |
| 13 | Vale of Lune 3rd XV | 35 | 21 | Leigh 2nd XV |
| 14 | Kirkby Lonsdale RUFC Development XV | v | Bye |
| 15 | West Park (St Helens) 2nd XV | v | Bye |
| 16 | Chester 3rd XV | 7 | 10 | Sale FC Vikings |
| Round 2 |
| Saturday, 21 October 2023 |
| Aldwinians 2nd XV | 20 | 21 | Eagle |
| Eccles 2nd XV | 102 | 0 | Garstang Blues (2nd XV) |
| Warrington 2nd XV | Away Walkover | Congleton |
| Burnage 2nd XV | Home Walkover | Sedgley Park 3rd XV |
| Vale of Lune 3rd XV | 24 | 12 | Kirkby Lonsdale RUFC Development XV |
| West Park (St Helens) 2nd XV | 10 | 50 | Sale FC Vikings |
| Saturday, 25 November 2023 |
| Liverpool St Helens 2nd XV | 33 | 34 | Crewe & Nantwich 2nd XV |
| Saturday, 9 December 2023 |
| Northwich 2nd XV | 14 | 17 | Didsbury Toc H 2nd XV |
| Round 3 |
| Saturday, 27 January 2024 |
| Eccles 2nd XV | 13 | 24 | Eagle |
| Didsbury Toc H 2nd XV | Home Walkover | Vale of Lune 3rd XV |
| Congleton | 32 | 31 | Burnage 2nd XV |
| Crewe & Nantwich 2nd XV | 10 | 29 | Sale FC Vikings |
| Round 4 |
| Saturday, 16 March 2024 |
| Didsbury Toc H 2nd XV | 25 | 14 | Eagle |
| Sale FC Vikings | 41 | 28 | Congleton |
| Round 5 |
| Saturday, 4 May 2024 |
| Didsbury Toc H 2nd XV | 27 | 17 | Sale FC Vikings |