| Round 1 |
| Saturday, 27 September 2025 |
| 9 | Northwich 2nd XV | v | Lymm Eagles |
| Saturday, 4 October 2025 |
| 1 | Oxton Parkonians | v | Birchfield |
| 2 | Chester 3rd XV | v | Caldy 3rd XV |
| 3 | Warrington 2nd XV | v | Vale of Lune 3rd XV |
| 4 | Liverpool University Veterinarians | v | Glossop 2nd XV |
| 5 | Newton-le-Willows | v | Crosby St Mary's |
| 6 | Trafford MV 2nd XV | v | Orrell 2nd XV |
| 7 | Preston Grasshoppers 4th XV | v | Bury 2nd XV |
| 8 | Port Sunlight | v | Sefton 2nd XV |
| 10 | Garstang Blues (2nd XV) | v | North Manchester |
| 11 | Moore | v | Heaton Moor 2nd XV |
| 12 | Blackburn 3rd XV | v | Eccles 3rd XV |
| 13 | Bolton 2nd XV | v | Altrincham Kersal Cougars (2nd XV) |
| 14 | Wilmslow 2nd XV | v | Winnington Park 2nd XV |
| 15 | Capenhurst | v | New Brighton 2nd XV |
| 16 | Macclesfield 3rd XV | v | Stockport RUFC 3rd XV Men |
| Round 2 |
| Saturday, 1 November 2025 |
| Winner of Match 1 | v | Winner of Match 2 |
| Winner of Match 3 | v | Winner of Match 4 |
| Winner of Match 5 | v | Winner of Match 6 |
| Winner of Match 7 | v | Winner of Match 8 |
| Winner of Match 9 | v | Winner of Match 10 |
| Winner of Match 11 | v | Winner of Match 12 |
| Winner of Match 13 | v | Winner of Match 14 |
| Winner of Match 15 | v | Winner of Match 16 |
| Round 3 |
| Saturday, 7 February 2026 |
| TBC | v | TBC |
| TBC | v | TBC |
| TBC | v | TBC |
| TBC | v | TBC |
| Round 4 |
| Saturday, 14 March 2026 |
| TBC | v | TBC |
| TBC | v | TBC |
| Round 5 |
| Saturday, 2 May 2026 |
| TBC | v | TBC |