| Round 1 |
| Saturday, 25 October 2025 |
| 1 | Widnes 2nd XV | 21 | 22 | Sandbach 2nd XV |
| Saturday, 1 November 2025 |
| 2 | Vale of Lune 2nd XV | Home Walkover | Anselmians 2nd XV |
| 3 | Eagle | v | Bye |
| 4 | Aldwinians 2nd XV | v | Bye |
| 5 | Blackburn 2nd XV | 45 | 12 | West Park (St Helens) 2nd XV |
| 7 | Birkenhead Park Wanderers (2nd XV) | v | Bye |
| 8 | Rochdale 2nd XV | v | Bye |
| Saturday, 6 December 2025 |
| 6 | Eccles 2nd XV | v | St Edward's O.B. |
| Round 2 |
| Saturday, 31 January 2026 |
| Sandbach 2nd XV | v | Vale of Lune 2nd XV |
| Saturday, 7 February 2026 |
| Eagle | v | Aldwinians 2nd XV |
| Blackburn 2nd XV | v | Winner of Match 6 |
| Birkenhead Park Wanderers (2nd XV) | v | Rochdale 2nd XV |
| Round 3 |
| Saturday, 7 March 2026 |
| TBC | v | TBC |
| TBC | v | TBC |
| Round 4 |
| Saturday, 2 May 2026 |
| TBC | v | TBC |