| Saturday, 6 September 2025 |
| Blackburn 2nd XV | v | Aldwinians 2nd XV |
| Sandbach 2nd XV | v | Eccles 2nd XV |
| St Edward's O.B. | v | Anselmians 2nd XV |
| Vale of Lune 2nd XV | v | Rochdale 2nd XV |
| West Park (St Helens) 2nd XV | v | Eagle |
| Widnes 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Saturday, 13 September 2025 |
| Anselmians 2nd XV | v | Sandbach 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | West Park (St Helens) 2nd XV |
| Eagle | v | St Edward's O.B. |
| Eccles 2nd XV | v | Aldwinians 2nd XV |
| Rochdale 2nd XV | v | Widnes 2nd XV |
| Vale of Lune 2nd XV | v | Blackburn 2nd XV |
| Saturday, 20 September 2025 |
| Aldwinians 2nd XV | v | Anselmians 2nd XV |
| Blackburn 2nd XV | v | Eccles 2nd XV |
| Sandbach 2nd XV | v | Eagle |
| St Edward's O.B. | v | Birkenhead Park Wanderers (2nd XV) |
| West Park (St Helens) 2nd XV | v | Rochdale 2nd XV |
| Widnes 2nd XV | v | Vale of Lune 2nd XV |
| Saturday, 4 October 2025 |
| Anselmians 2nd XV | v | Eccles 2nd XV |
| Eagle | v | Aldwinians 2nd XV |
| Rochdale 2nd XV | v | St Edward's O.B. |
| Sandbach 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Vale of Lune 2nd XV | v | West Park (St Helens) 2nd XV |
| Widnes 2nd XV | v | Blackburn 2nd XV |
| Saturday, 11 October 2025 |
| Aldwinians 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Blackburn 2nd XV | v | Anselmians 2nd XV |
| Eccles 2nd XV | v | Eagle |
| Sandbach 2nd XV | v | Rochdale 2nd XV |
| St Edward's O.B. | v | Vale of Lune 2nd XV |
| West Park (St Helens) 2nd XV | v | Widnes 2nd XV |
| Saturday, 18 October 2025 |
| Birkenhead Park Wanderers (2nd XV) | v | Eccles 2nd XV |
| Eagle | v | Anselmians 2nd XV |
| Rochdale 2nd XV | v | Aldwinians 2nd XV |
| Vale of Lune 2nd XV | v | Sandbach 2nd XV |
| West Park (St Helens) 2nd XV | v | Blackburn 2nd XV |
| Widnes 2nd XV | v | St Edward's O.B. |
| Saturday, 25 October 2025 |
| Aldwinians 2nd XV | v | Vale of Lune 2nd XV |
| Anselmians 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Blackburn 2nd XV | v | Eagle |
| Eccles 2nd XV | v | Rochdale 2nd XV |
| St Edward's O.B. | v | West Park (St Helens) 2nd XV |
| Widnes 2nd XV | v | Sandbach 2nd XV |
| Saturday, 8 November 2025 |
| Birkenhead Park Wanderers (2nd XV) | v | Eagle |
| Rochdale 2nd XV | v | Anselmians 2nd XV |
| St Edward's O.B. | v | Blackburn 2nd XV |
| Vale of Lune 2nd XV | v | Eccles 2nd XV |
| West Park (St Helens) 2nd XV | v | Sandbach 2nd XV |
| Widnes 2nd XV | v | Aldwinians 2nd XV |
| Saturday, 22 November 2025 |
| Aldwinians 2nd XV | v | West Park (St Helens) 2nd XV |
| Anselmians 2nd XV | v | Vale of Lune 2nd XV |
| Blackburn 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Eagle | v | Rochdale 2nd XV |
| Eccles 2nd XV | v | Widnes 2nd XV |
| Sandbach 2nd XV | v | St Edward's O.B. |
| Saturday, 29 November 2025 |
| Rochdale 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Sandbach 2nd XV | v | Blackburn 2nd XV |
| St Edward's O.B. | v | Aldwinians 2nd XV |
| Vale of Lune 2nd XV | v | Eagle |
| West Park (St Helens) 2nd XV | v | Eccles 2nd XV |
| Widnes 2nd XV | v | Anselmians 2nd XV |
| Saturday, 6 December 2025 |
| Aldwinians 2nd XV | v | Sandbach 2nd XV |
| Anselmians 2nd XV | v | West Park (St Helens) 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Vale of Lune 2nd XV |
| Blackburn 2nd XV | v | Rochdale 2nd XV |
| Eagle | v | Widnes 2nd XV |
| Eccles 2nd XV | v | St Edward's O.B. |
| Saturday, 13 December 2025 |
| Aldwinians 2nd XV | v | Eccles 2nd XV |
| Blackburn 2nd XV | v | Vale of Lune 2nd XV |
| Sandbach 2nd XV | v | Anselmians 2nd XV |
| St Edward's O.B. | v | Eagle |
| West Park (St Helens) 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Widnes 2nd XV | v | Rochdale 2nd XV |
| Saturday, 20 December 2025 |
| Anselmians 2nd XV | v | Aldwinians 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | St Edward's O.B. |
| Eagle | v | Sandbach 2nd XV |
| Eccles 2nd XV | v | Blackburn 2nd XV |
| Rochdale 2nd XV | v | West Park (St Helens) 2nd XV |
| Vale of Lune 2nd XV | v | Widnes 2nd XV |
| Saturday, 10 January 2026 |
| Aldwinians 2nd XV | v | Eagle |
| Birkenhead Park Wanderers (2nd XV) | v | Sandbach 2nd XV |
| Blackburn 2nd XV | v | Widnes 2nd XV |
| Eccles 2nd XV | v | Anselmians 2nd XV |
| St Edward's O.B. | v | Rochdale 2nd XV |
| West Park (St Helens) 2nd XV | v | Vale of Lune 2nd XV |
| Saturday, 17 January 2026 |
| Anselmians 2nd XV | v | Blackburn 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Aldwinians 2nd XV |
| Eagle | v | Eccles 2nd XV |
| Rochdale 2nd XV | v | Sandbach 2nd XV |
| Vale of Lune 2nd XV | v | St Edward's O.B. |
| Widnes 2nd XV | v | West Park (St Helens) 2nd XV |
| Saturday, 31 January 2026 |
| Aldwinians 2nd XV | v | Rochdale 2nd XV |
| Anselmians 2nd XV | v | Eagle |
| Blackburn 2nd XV | v | West Park (St Helens) 2nd XV |
| Eccles 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| Sandbach 2nd XV | v | Vale of Lune 2nd XV |
| St Edward's O.B. | v | Widnes 2nd XV |
| Saturday, 14 February 2026 |
| Birkenhead Park Wanderers (2nd XV) | v | Anselmians 2nd XV |
| Eagle | v | Blackburn 2nd XV |
| Rochdale 2nd XV | v | Eccles 2nd XV |
| Sandbach 2nd XV | v | Widnes 2nd XV |
| Vale of Lune 2nd XV | v | Aldwinians 2nd XV |
| West Park (St Helens) 2nd XV | v | St Edward's O.B. |
| Saturday, 28 February 2026 |
| Aldwinians 2nd XV | v | Widnes 2nd XV |
| Anselmians 2nd XV | v | Rochdale 2nd XV |
| Blackburn 2nd XV | v | St Edward's O.B. |
| Eagle | v | Birkenhead Park Wanderers (2nd XV) |
| Eccles 2nd XV | v | Vale of Lune 2nd XV |
| Sandbach 2nd XV | v | West Park (St Helens) 2nd XV |
| Saturday, 14 March 2026 |
| Birkenhead Park Wanderers (2nd XV) | v | Blackburn 2nd XV |
| Rochdale 2nd XV | v | Eagle |
| St Edward's O.B. | v | Sandbach 2nd XV |
| Vale of Lune 2nd XV | v | Anselmians 2nd XV |
| West Park (St Helens) 2nd XV | v | Aldwinians 2nd XV |
| Widnes 2nd XV | v | Eccles 2nd XV |
| Saturday, 21 March 2026 |
| Aldwinians 2nd XV | v | St Edward's O.B. |
| Anselmians 2nd XV | v | Widnes 2nd XV |
| Birkenhead Park Wanderers (2nd XV) | v | Rochdale 2nd XV |
| Blackburn 2nd XV | v | Sandbach 2nd XV |
| Eagle | v | Vale of Lune 2nd XV |
| Eccles 2nd XV | v | West Park (St Helens) 2nd XV |
| Saturday, 28 March 2026 |
| Rochdale 2nd XV | v | Blackburn 2nd XV |
| Sandbach 2nd XV | v | Aldwinians 2nd XV |
| St Edward's O.B. | v | Eccles 2nd XV |
| Vale of Lune 2nd XV | v | Birkenhead Park Wanderers (2nd XV) |
| West Park (St Helens) 2nd XV | v | Anselmians 2nd XV |
| Widnes 2nd XV | v | Eagle |
| Saturday, 11 April 2026 |
| Aldwinians 2nd XV | v | Blackburn 2nd XV |
| Anselmians 2nd XV | v | St Edward's O.B. |
| Birkenhead Park Wanderers (2nd XV) | v | Widnes 2nd XV |
| Eagle | v | West Park (St Helens) 2nd XV |
| Eccles 2nd XV | v | Sandbach 2nd XV |
| Rochdale 2nd XV | v | Vale of Lune 2nd XV |